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CBL218

Anti-Keratin K3/K76 Antibody, clone AE5

clone AE5, Chemicon®, from mouse

Synonym(s):

Anti-HUMCYT2A, Anti-KRT2P

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
AE5, monoclonal
Application:
IHC (p), WB
Citations:
21
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biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

AE5, monoclonal

species reactivity

human, mouse, bovine, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable

isotype

IgG1

NCBI accession no.

NM_057088.2

UniProt accession no.

P12035, Q01546

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... KRT3(3850)

General description

64 kDa

Immunogen

Rabbit corneal epithelial keratin

Application

Anti-Keratin K3/K76 Antibody, clone AE5 is an antibody against Keratin K3/K76 for use in WB, IH, IH(P).
Immunohistochemistry on frozen sections: 1:50.

Immunohistochemistry on Paraffin-embedded sections: 1:50 on paraffin-embedded tissue sections fixed in 10% neutral buffered formalin or Fekete’s acid-alcohol-formalin following trypsin digestion antigen retrieval (Mikaelian et al., 2004).

Western blotting: 1:500.

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

The antibody reacts with the 64 kDa polypeptide (cytokeratin 3; Keratin K3) of corneal epithelium and keratin K76 (cytokeratin K2p) of palate epithelium. As such this represents an excellent marker for studying corneal epithelial-type differentiation. Also recognizes bovine snout and lip epithelium, as well as a rabbit corneal cell line.

Physical form

Format: Purified
The monoclonal was purified by ion exchange chromatography is presented in phosphate buffered saline (pH 7.2) containing 0.09% sodium azide.

Preparation Note

For use within 1 month of purchase store at 2°-8°C, for long term storage aliquot antibody into small volumes and store at -20°C for up to one year.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Storage Class

12 - Non Combustible Liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A biomimetic scaffold for culturing limbal stem cells: a promising alternative for clinical transplantation.
Subhadra Dravida, Subhash Gaddipati, May Griffith, Kim Merrett, Soundarya Lakshmi Madhira et al.
Journal of Tissue Engineering and Regenerative Medicine null
Xiaojun Lian et al.
PloS one, 8(3), e60016-e60016 (2013-03-26)
Human pluripotent stem cells (hPSCs) provide unprecedented opportunities to study the earliest stages of human development in vitro and have the potential to provide unlimited new sources of cells for regenerative medicine. Although previous studies have reported cytokeratin 14+/p63+ keratinocyte
Differentiation-related expression of a major 64K corneal keratin in vivo and in culture suggests limbal location of corneal epithelial stem cells.
Schermer, A; Galvin, S; Sun, TT
The Journal of cell biology null
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Global Trade Item Number

SKUGTIN
CBL21804053252361777