MAB1957
Anti-Integrin beta 3 (ITGB3/CD61) Antibody
CHEMICON®, mouse monoclonal, 25E11
Synonym(s):
CD61
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
25E11, monoclonal
Species reactivity:
human
Application:
FACS, ICC, IP
Citations:
40
Product Name
Anti-Integrin β3 Antibody, clone 25E11, clone 25E11, Chemicon®, from mouse
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
25E11, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgM
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGB3(3690)
Immunogen
Normal blood mononuclear cells activated by mixed lymphocyte reaction and cultured for 10 days in medium containing IL-2.
Application
Immunoprecipitation
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
Immunocytochemistry (acetone fixation required)
Flow cytometry
Inhibits platelet aggregation, 10 μg/mL
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β3 Antibody, clone 25E11 is validated for use in FC, IP, IC, FUNC for the detection of Integrin β3.
Biochem/physiol Actions
Reacts with human integrin beta3 (GPIIIa, vitronectin receptor beta chain). The protein detectable is a complex of CD41 and CD61. The apparent molecular weight of the GPIIIa by SDS-PAGE is 105 kDa reduced and 90 kDa unreduced. Ligands are fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin. Residues 237-248 of GPIIIa or CD61 are critical in adhesive protein binding. The monocytoid cell line U937 is highly positive.
(original paper claimed IgG2ak)
(original paper claimed IgG2ak)
Physical form
Format: Purified
In buffer of 0.02M phosphate, 0.25M sodium chloride, pH 7.6, 0.1% sodium azide.
Preparation Note
Maintain between 2 and 8°C.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-1060
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Storage Class
12 - Non Combustible Liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Francisco M Vega et al.
Open biology, 2(5), 120076-120076 (2012-06-23)
The Rho GTPase RhoB has been shown to affect cell migration, but how it does this is not clear. Here we show that cells depleted of RhoB by RNAi are rounded and have defects in Rac-mediated spreading and lamellipodium extension
[Laminin alpha3 chain-derived peptide promotes keratinocyte migration and wound closure: clustering of syndecan-4 and integrin beta1].
Atsushi Utani
Seikagaku. The Journal of Japanese Biochemical Society null
Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived peptide promotes keratinocyte migration.
Eri Araki, Yutaka Momota, Takeshi Togo, Miki Tanioka, Kentaro Hozumi, Motoyoshi Nomizu et al.
Molecular Biology of the Cell null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB1957 | 04053252666940 |