Anti-Nestin Antibody, clone rat-401, Alexa Fluor^™ 488 conjugate

Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells (Mokry and Nemecek, 1998). Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinomas.

The unconjugated parent antibody (Catalog No. MAB353) has an observed MW at 220-240 kDa

Embryonic rat spinal cord

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

Research Category
Stem Cell Research

Neuroscience

Detect the Nestin protein using this Anti-Nestin, clone rat-401, Alexa Fluor 488 conjugate validated for use in ICC.

Evaluated by Immunocytochemistry in rat hippocampal neural stem cells (SCR022). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in rat hippocampal neural stem cells (SCR022).

This antibody recognizes both rat and mouse Nestin. Antibody is not reactive with human Nestin protein.

Protein G Purified

Purified mouse monoclonal IgG1 conjugated to Alexa Fluor^™ 488 in PBS with 0.1% sodium azide and 15mg/ml BSA.

Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Control
Mouse cortical neural stem cells (SCR029)

Evaluated by Immunocytochemistry in mouse cortical neural stem cells (SCR029). Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Nestin in mouse cortical neural stem cells (SCR029).

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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