Anti-Vesicular Glutamate Transporter 1 Antibody

60 kDa

VGLUT1 is expressed in a subset of glutamate neurons and transports glutamate into native synaptic vesicles from the brain, exhibiting a conductance for chloride that is blocked by glutamate. Vesicular glutamate transport has a substantially lower apparent affinity than the plasma membrane excitatory amino acid transporters. Glutamate transport by VGLUT1 is saturated with a K(m) of approximately 2 mM, in the same range as transport by synaptic vesicles. Finally, plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, whereas VGLUT1 does not recognize aspartate. Vesicular glutamate transporters pack the neurotransmitter into synaptic vesicles so that they can be released into the synapse. VGLUTs are dependent on a proton gradient that they create by hydrolysing adenosine triphosphate (ATP). VGLUTs have only between one hundredth and one thousandth the affinity for glutamate that EAATs have.

Recombinant protein from rat VGLUT1.

Immunohistochemistry:
A previous lot of this antibody was tested on IH.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Neuronal & Glial Markers

This Anti-Vesicular Glutamate Transporter 1 Antibody, clone 3C10.2, Cat. No. MAB5502, is validated for use in IH, WB for the detection of VGluT1.

Reacts with VGLUT1 (Vesicular Glutamate Transporter 1).

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.1% sodium azide.

Stable for 6 months at 2-8ºC in undiluted aliquots from date of receipt.

Control
Brain

VGLUT1 Control Peptide AG208

Routinely evaluated by Western Blot on Mouse Brain lysates.

Western Blot Analysis:
1:500 dilution of this lot detected Vesicular Glutamate Transporter 1 on 10 μg of Mouse Brain lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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