Anti-Cytomegalovirus Antibody, clone 8B1.2

68-72 kDa

Human cytomegalovirus (HCMV) is a ubiquitous human pathogen that belongs to the herpes adenovirus family. The viral life cycle takes approximately seventy two hours. After the initial fusion of the viral envelope with the plasma membrane of the cell, the encapisidated virus particle is released into the cytoplasm and within minutes, transits to the nucleus. Via active transport through the nuclear pore, the capsid gains entry and viral DNA is deposited. Viral gene expression then occurs in a temporally regulated manner, first with expression of the immediate early genes, followed by the early genes, then, after viral replication has commenced, the late genes. All of the immediate early proteins have been shown to be transactivators, with IE1-72 and IE2-86 being the most well characterized. These genes regulate the expression of factors required for virus replication.

Affinity purified immediate early antigen from MRC-5 cells infected with CMV AD169 (ATCC).

Anti-Cytomegalovirus Antibody, clone 8B1.2 detects level of Cytomegalovirus & has been published & validated for use in ELISA, FC, IF, IH(P) & WB.

ELISA

Western Blot: 1:200. Recognizes approximately a 70kDa band.

Immunofluorescence: 1:200-1:400.

Immunohistochemistry 1:200-1:800.

Works on paraffin embedded tissue sections.

FACS

Optimal working dilutions must be determined by end user.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Reacts with an immediate early non-structural antigen of 68-72 kDa. This antigen can be detected 1 hour after infection exhibiting an intranuclear staining pattern. This staining reaches a peak at 10-12 hours. This antigen persists and can be detected throughout the complete CMV infection cycle.

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Cytomegalovirus Control Slides, Catalogue Number 5027-5

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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