MABD51
Anti-Brn-2 (POU3F2) Antibody, clone 8C4.2
clone 8C4.2, from mouse
Synonym(s):
POU domain, class 3, transcription factor 2, Brain-specific homeobox/POU domain protein 2, Brain-2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Oct-7, Octamer-binding transcription factor 7, OTF
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
8C4.2, monoclonal
Application:
IHC, WB
Citations:
5
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
8C4.2, monoclonal
species reactivity
rat, human
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... POU3F2(5454)
General description
Brn-2 (POU domain, class 3, transcription factor 2; Oct-7; N-Oct-3; or POU3F2) is a member of a large family of POU-domain transcription factors. The highly homologous POU domain contains a POU-specific domain at the N-terminus, a POU-homeo domain at the C-terminus and an interconnecting linker region. The POU domain binds to the DNA sequence 5’-ATGCAAAT-3’. Brn-2 is highly expressed in the developing CNS and may play a role in neurogenesis, particularly in the development of the hypothalamus. Previous studies have also suggested that Brn-2 integrates signals downstream of the BRAF/MAP kinase and Wnt/β-catenin pathways, and Brn-2 may be involved in the transformation and proliferation of melanomas.
~54 kDa observed.
The calculated molecular weight is 47 kDa Brn-2 (POU3F2) may be observed at ~50-55 kDa in some cell lysates (Wolfe, A., et al., (2002). Molecular Endocrinology. 16(3):435-449).
The calculated molecular weight is 47 kDa Brn-2 (POU3F2) may be observed at ~50-55 kDa in some cell lysates (Wolfe, A., et al., (2002). Molecular Endocrinology. 16(3):435-449).
Immunogen
GST-tagged recombinant protein corresponding human Brn-2 (POU3F2).
Application
Anti-Brn-2 (POU3F2) Antibody, clone 8C4.2 detects level of Brn-2 (POU3F2) & has been published & validated for use in Western Blotting, IHC(P).
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Brn-2 in normal rat brain tissue, in Purkinje, basket, and glial cells of rat cerebellum tissue, in neurons of rat cerebellum tissue, in neurons and glial cells of rat frontal cortex tissue, and in neurons of human pons tissue.
Physical form
Format: Purified
Analysis Note
Control
SK-N-MC cell lysates
SK-N-MC cell lysates
Evaluated by Western Blot in SK-N-MC cell lysates.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Brn-2 (POU3F2) in 10 µg of SK-N-MC cell lysates.
Western Blot Analysis: A 1:2,000 dilution of this antibody detected Brn-2 (POU3F2) in 10 µg of SK-N-MC cell lysates.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Zhongyuan Bao et al.
Oxidative medicine and cellular longevity, 2021, 6338722-6338722 (2021-12-03)
Traumatic brain injury (TBI) causes a high rate of mortality and disability, and its treatment is still limited. Loss of neurons in damaged area is hardly rescued by relative molecular therapies. Based on its disease characteristics, we transplanted human embryonic
Meitetsu Masawa et al.
The American journal of pathology, 192(6), 847-861 (2022-04-04)
Although recent reports have revealed the importance of the inactivation of both RB1 and TP53 in the transformation from lung adenocarcinoma into neuroendocrine carcinoma (NEC), the requirements for complete transformation into NEC have not been elucidated. To investigate alterations in
Aaron Gordon et al.
Nature neuroscience, 24(3), 331-342 (2021-02-24)
Human stem-cell-derived models provide the promise of accelerating our understanding of brain disorders, but not knowing whether they possess the ability to mature beyond mid- to late-fetal stages potentially limits their utility. We leveraged a directed differentiation protocol to comprehensively
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABD51 | 04053252438110 |