Anti-SMARCA4 Antibody, clone 20C3.2

Also known as Protein BRG1 and SNF2-beta, among other aliases, SMARCA4 a transcriptional activator belonging to the SNF2/RAD54 helicase family, and acts as a component of the SWI/SNF ATP-dependent chromatin remodeling complexes family. BRG1 contains 4 domains of interest; a bromo domain, a helicase ATP-binding domain, a helicase C-terminal domain, and a HAS domain. It is a component of several different complexes with distinctive roles such as the CREST-BRG1 complex which regulates the activation of promoters in a calcium-dependent manner, releasing repressor complexes and recruiting activator complexes. It is also a part of the nBAF and npBAF complexes which are involved in the chromatin remodeling of mononucleosomes and nucleosomal arrays. As a component of the SWI/SNF complex, SMARCA4 has a role in tumor suppression and has been shown to regulate cell cycle and differentiation when present in neoplasma.

~184 kDa observed

GST-tagged recombinant protein corresponding to the human SMARCA4.

Anti-SMARCA4 Antibody, clone 20C3.2 is a Mouse Monoclonal Antibody for detection of SMARCA4 also known as Transcription activator BRG1, SNF2-beta & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in PC-12 cell lysate.

Immunocytochemistry Analysis: 1 µg/mL of this antibody detected SMARCA4 in HeLa and A431 cells.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa nuclear extract

Evaluated by Western Blot in HeLa nuclear extract.

Western Blot Analysis: 0.5 µg/mL of this antibody detected SMARCA4 in 10 µg of HeLa nuclear extract.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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