MABE867
Anti-Mad1 Antibody, clone BB3-8
clone BB3-8, from mouse
Synonym(s):
Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
BB3-8, monoclonal
Application:
IF, WB
Citations:
8
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BB3-8, monoclonal
species reactivity
human
technique(s)
immunofluorescence: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MAD1L1(8379)
General description
Mitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20.
~83 kDa observed
Immunogen
His-tagged recombinant protein corresponding to human Mad1.
Application
Anti-Mad1 Antibody, clone BB3-8 is a highly specific mouse monoclonal antibody, that targets MAD1 & has been tested in western blotting & Immunofluorescence.
Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Immunoflourescence Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cells (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Mad1 in HeLa cell lysate (Screpanti, E., et al. (2011). Curr Biol. 21(5):391-398.).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Mad1 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Emanuela Screpanti et al.
Current biology : CB, 21(5), 391-398 (2011-03-01)
Kinetochores are proteinaceous scaffolds implicated in the formation of load-bearing attachments of chromosomes to microtubules during mitosis. Kinetochores contain distinct chromatin- and microtubule-binding interfaces, generally defined as the inner and outer kinetochore, respectively (reviewed in). The constitutive centromere-associated network (CCAN)
Stefano Santaguida et al.
The EMBO journal, 30(8), 1508-1519 (2011-03-17)
Fidelity of chromosome segregation is ensured by a tension-dependent error correction system that prevents stabilization of incorrect chromosome-microtubule attachments. Unattached or incorrectly attached chromosomes also activate the spindle assembly checkpoint, thus delaying mitotic exit until all chromosomes are bioriented. The
Lindsey A Allan et al.
The EMBO journal, 39(12), e103180-e103180 (2020-03-24)
Cyclin B:CDK1 is the master kinase regulator of mitosis. We show here that, in addition to its kinase functions, mammalian Cyclin B also scaffolds a localised signalling pathway to help preserve genome stability. Cyclin B1 localises to an expanded region
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MABE867 | 04053252921018 |