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ST1704

Anti-TOMM22 Mouse mAb (4G4)

Name: Anti-TOMM22 Mouse mAb (4G4)
Brand: MM

liquid, clone 4G4, Calbiochem^®

Synonym(s):

Anti-Translocase of Outer Mitochondrial Membrane 22

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About This Item

NACRES:

NA.41

UNSPSC Code:

12352203

Clone:

4G4, monoclonal

Species reactivity:

human, rat, mouse

Application:

—

Citations:

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Properties

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4G4, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, rat, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG2a

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TOMM22(56993)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~18 kDa TOMM22 protein.

Recognizes the ~18 kDa TOMM22 protein in PC-12, NIH3T3, Raw 264.7, and HeLa cells and human small intestine tissue.

This Anti-TOMM22 Mouse mAb (4G4) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM22.

Immunogen

Full-length, recombinant, human TOMM22 (aa 1-143), expressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
PC 12 cells, NIH3T3 cells, Raw 264.7 cells, HeLa cells, Human small intestine tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c