Key Documents

View All Documentation

ST1705

Anti-TOMM20 Mouse mAb (4F3)

Name: Anti-TOMM20 Mouse mAb (4F3)
Brand: MM

liquid, clone 4F3, Calbiochem^®

Synonym(s):

Anti-Translocase of Outer Mitochondrial Membrane 20

Select a Size

Change View

About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

4F3, monoclonal

Species reactivity:

human, rat, mouse

Application:

—

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

4F3, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, rat, mouse

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (3 µg/mL))

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TOMM20(9804)

Description

General description

Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.

Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.

This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.

Immunogen

Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

Still not finding the right product?

Try our Product Selector Tool to narrow your options

Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c