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10109223001

Roche

RNA from yeast

dry powder, pkg of 100 g

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Synonym(s):
RNA from yeast, RNA

biological source

yeast

Quality Level

100

form

dry powder

packaging

pkg of 100 g

manufacturer/tradename

Roche

color

yellowish to brown

solubility

water: soluble

storage temp.

room temp

Related Categories

General description

RNA from yeast is the total RNA obtained from Candida utilis.

Application

RNA from yeast has been used:
  • as a carrier RNA for in situ hybridization
  • for RNA coimmunoprecipitation
RNA from yeast has been used:
  • to investigate its effect on the formation of transactivation response element (TAR) DNA-binding protein 43 (TDP-43) low-complexity domain (LCD) droplets in phosphate buffer to determine the possible influence of RNA binding on the liquid–liquid phase separation (LLPS) and/or aggregation of TDP-43 LCD
  • for biomass fractionation to help with separations to assess the fate of methylphosphonate (MPn) or bicarbonate radiotracers in cells
  • in hybridization solution for in situ hybridization

Preparation Note

Working solution: This mixture of total yeast RNA is resuspended with sterile, DEPC-treated double-distilled water or TE buffer to 10 mg/ml if used as carrier.

Analysis Note

Absorbance: RNA: 95%, Pi (phosphate): 0.3%

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Britta Jedamzik et al.
Cold Spring Harbor protocols, 2009(10), pdb-pdb (2010-02-12)
RNA coimmunoprecipitation (co-IP) experiments are an extension of protein co-IP experiments in which in vivo RNA-protein complexes are investigated. This protocol describes how to perform RNA co-IPs from C. elegans whole-worm extracts. In principle, a protein-specific antibody is used to
Alexandre Paix et al.
Methods in molecular biology (Clifton, N.J.), 714, 49-70 (2011-03-25)
In several species, axis formation and tissue differentiation are the result of developmental cascades which begin with the localization and translation of key maternal mRNAs in eggs. Localization and anchoring of mRNAs to cortical structures can be observed with high

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