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DUTP-RO

Roche

dUTP

PCR Grade, sodium salt

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Synonym(s):
2′-Deoxyuridine 5′-triphosphate sodium salt solution, dUTP sodium salt
Empirical Formula (Hill Notation):
C9H15N2O14P3 · xNa+
CAS Number:
94736-09-1
Molecular Weight:
468.14 (free acid basis)
MDL number:
MFCD01630759
PubChem Substance ID:
329798931

Quality Level

100

Assay

99% (dNTP)

form

solution

usage

sufficient for 125,000 standard reactions (03732720001)
 sufficient for 31,250 standard reactions (11969056001)
 sufficient for 6,250 standard reactions (11934554001)

packaging

pkg of 1,250 μL (11969056001 [100 mM])
pkg of 4 × 1,250 μL (03732720001 [4x100 mM])
pkg of 250 μL (11934554001 [100 mM])

manufacturer/tradename

Roche

concentration

<0.9% (dNDP)
100 mM

storage temp.

−20°C

SMILES string

[Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O

InChI

1S/C9H15N2O14P3.Na.H/c12-5-3-8(11-2-1-7(13)10-9(11)14)23-6(5)4-22-27(18,19)25-28(20,21)24-26(15,16)17;;/h1-2,5-6,8,12H,3-4H2,(H,18,19)(H,20,21)(H,10,13,14)(H2,15,16,17);;

InChI key

FVFWWPFKJNTHIQ-UHFFFAOYSA-N

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General description

dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Application

dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dUTP water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP (each) and 30 μl dUTP to 40 μl Water,PCR grade).

Other Notes

For general laboratory use.
PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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K N Fukui et al.
Plant & cell physiology, 42(2), 189-196 (2001-03-07)
Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon. FISH analysis using a large DNA insert library of wheat identified a 67-kb clone (R11H) that showed strong hybridization signals on the centromeres. The R11H clone contains
Mario Hermsen et al.
Gastroenterology, 123(4), 1109-1119 (2002-10-03)
Current models of colorectal adenoma to carcinoma progression do not fully reflect the genetic heterogeneity and complexity of the disease. The aim of the present study was to identify genetic changes discriminating adenomas that have progressed to carcinoma from adenomas

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