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AGRMP-RO

Roche

Agarose MP

powder, pkg of 100 g (11388983001), pkg of 500 g (11388991001)

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Synonym(s):
agarose

description

multipurpose agarose

form

powder

packaging

pkg of 100 g (11388983001)
pkg of 500 g (11388991001)

manufacturer/tradename

Roche

storage temp.

20-25°C

Related Categories

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose MP, a multi purpose agarose, is developed for analytical and preparative electrophoresis of nucleic acids and separation of high molecular weight DNA via pulse field gel electrophoresis (PFGE).It is also used for preparative electrophoresis and quantitative recovery of biologically active DNA. Agarose MP has also been used along with other supplements to store embryos in different developmental stages.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

Agarose MP can produce high strength gels (e.g., the gel strength of a 1% agarose MP gel is ?1800 g/cm2). Even gels that have low concentrations of agarose MP are strong enough to be used for electrophoresis. The agarose matrix of such low concentration gels allows high molecular weight molecules to migrate rapidly without significant restriction.
Agarose MP is particularly well suited for pulsed-field gel electrophoresis, because it allows short separation times for large DNA molecules, while maintaining the high resolution required for effective analysis.
For life science research only. Not for use in diagnostic procedures.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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Pei Yun Lee et al.
Journal of visualized experiments : JoVE, 62(62), 3923-3923 (2012-05-02)
Agarose gel electrophoresis is the most effective way of separating DNA fragments of varying sizes ranging from 100 bp to 25 kb(1). Agarose is isolated from the seaweed genera Gelidium and Gracilaria, and consists of repeated agarobiose (L- and D-galactose)
François Sipieter et al.
PloS one, 10(10), e0140924-e0140924 (2015-10-31)
Uncoupling of ERK1/2 phosphorylation from subcellular localization is essential towards the understanding of molecular mechanisms that control ERK1/2-mediated cell-fate decision. ERK1/2 non-catalytic functions and discoveries of new specific anchors responsible of the subcellular compartmentalization of ERK1/2 signaling pathway have been

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