198161
Toluidine Blue O
certified by the BSC
Synonym(s):
Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride
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About This Item
Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
EC Number:
202-146-2
Colour Index Number:
52040
MDL number:
agency
certified by the BSC
Quality Level
form
powder
composition
Dye content, 80%
color
dark green
λmax
626 nm
ε (extinction coefficient)
≥13000 at 241-247 nm in H2O, ≥28000 at 287-293 nm in H2O, ≥40000 at 625-635 nm in H2O
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C
InChI
1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H
InChI key
GEDVVYWLPUPJJZ-UHFFFAOYSA-N
General description
A metachromatic dye suitable for a wide variety of histological staining procedures.
Application
Toluidine Blue O along with light-emitting diode has been used as a photosensitizer to control in vitro biofilms and to kill gram-negative bacteria.
Certified for use as a metachromatic stain for mast cells and cartilage using the Churukian-Schenk method and the method of Nocht on paraffin sections.
Biochem/physiol Actions
Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. TBO can be removed from its aqueous solution by using an effective adsorbent, clinoptilolite. TBO and fixed output laboratory laser light source are used together to destroy variety of oral bacteria associated with dental caries. This metachromatic nuclear stain is often used in place of thionin or methylene blue. It also serves as a component of a tribasic stain along with Malachite Greenand Basic Fuchsin.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The adsorption kinetics and removal of cationic dye, Toluidine Blue O, from aqueous solution with Turkish zeolite
Alpat S K
Journal of Hazardous Materials, 151(1), 213-220 (2008)
A TRIBASIC STAIN FOR THIN SECTIONS OF PLASTIC-EMBEDDED, OSO4-FIXED TISSUES.
P M GRIMLEY
Stain technology, 39, 229-233 (1964-07-01)
Factors influencing the susceptibility of Gram-negative bacteria to toluidine blue O-mediated lethal photosensitization
komerik N
Journal of Applied Microbiology, 92(4), 618?623-618?623 (2002)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 198161-25G | 04061838762757 |
| 198161-5G | 04061838762764 |