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Nutrient Agar

suitable for microbiology, NutriSelect® Plus

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Synonym(s):
NA, standard nutrient agar
NACRES:
NA.74

Agency

according to ISO 16266:2006

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
meat extract, 1 g/L
peptone, 5 g/L
sodium chloride, 5 g/L
yeast extract, 2 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.1±0.2 (25 °C)

application(s)

agriculture
clinical testing
environmental
food and beverages
life science and biopharma
water monitoring
microbiology

storage temp.

2-25°C

suitability

nonselective for Aspergillus spp.
nonselective for Bacillus spp.
nonselective for Candida spp.
nonselective for Enterococcus spp.
nonselective for Escherichia coli
nonselective for Listeria spp.
nonselective for Pseudomonas spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Nutrient medium is a general basic culture medium for less fastidious microorganisms. Meat extract, yeast extract and peptone provide nitrogen, vitamins, amino acids and carbon for growth. Sodium Chloride ensure osmotic balance. Thus, when adding blood, as supplement the blood cells will not rupture. Addition of 10% different biological fluids such as blood, serum and egg yolk makes it suitable for the cultivation of related fastidious organism. Acc. to Gray et al. the addition of 0.05% potassium tellurite makes the Nutrient medium an excellent enrichment medium for Listeria monocytogenes.

Application

  • Nutrient Agar is used for the routine cultivation of microorganisms not requiring specific nutritional requirements
  • It is also used for the enumeration and enrichment of bacteria
  • Nutrient Agar can be used as a base for preparing special culture media.

Preparation Note

Suspend 28 g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies on heterotrophic bacteria in some Argentine soils

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物来源培养基

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M.A. Sagardoy et al.
Anal. Edaf. Agrobiol., 42, 2069-2069 (1984)
Wei Zhao et al.
Journal of cellular biochemistry, 120(10), 17337-17344 (2019-06-19)
Transcriptional coactivator with PDZ-binding motif (TAZ), a Hippo pathway downstream effector, promotes tumor progression by serving as a transcriptional coactivator with TEAD. Here, we introduced a new construct which can express the TEAD-binding domain of TAZ protein (TAZBD), and determined
Laura E McMillan et al.
The Journal of experimental biology, 223(Pt 19) (2020-10-14)
For many animals, the gut microbiome plays an essential role in immunity and digestion. However, certain animals, such as the caterpillar Manduca sexta, do not have a resident gut microbiome. Although these animals do have bacteria that pass through their

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