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77187

Buffered Peptone Water

NutriSelect® Plus, powder, pack of 500g or 25kg, pack of 500 g or 25 kg, non-sterile

Synonym(s):

BPW, Buffered Peptone Water, Tryptone Phosphate Water

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About This Item

NACRES:
NA.85
UNSPSC Code:
41171607
Manufacturer/tradename:
NutriSelect® Plus
Agency:
according to GB 4789.30-2016, according to GB 4789.40-2016, according to ISO 21528-1:2017, according to ISO 22964:2017
Form:
powder
Suitability:
Providencia spp., nonselective for Citrobacter spp., nonselective for Cronobacter spp., nonselective for Escherichia coli, nonselective for Klebsiella spp., nonselective for Proteus spp., nonselective for Pseudomonas spp., nonselective for Salmonella spp., nonselective for Shigella spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for Yersinia spp., nonselective for bacteria (General Media), nonselective for coliforms, nonselective for enterobacteriaceae
Application(s):
environmental
food and beverages
microbiology
pathogen testing
sample preparation
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Quality Level

200

agency

according to GB 4789.30-2016, according to GB 4789.40-2016, according to ISO 21528-1:2017, according to ISO 22964:2017

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

disodium hydrogen phosphate, 3.5 g/L , peptone, 10 g/L , potassium dihydrogen phosphate, 1.5 g/L , sodium chloride, 5 g/L

manufacturer/tradename

NutriSelect® Plus

packaging

pack of 500 g or 25 kg, pack of 500g or 25kg

technique(s)

microbe id | specific enzyme detection: suitable, microbiological culture: suitable

color

faintly beige Powder, Homogeneous, free-flowing powder, clear faintly brownish-yellow Prepared media, without any precipitate

final pH

7.2±0.2 (25 °C)

application(s)

environmental
food and beverages
microbiology
pathogen testing
sample preparation

suitability

Providencia spp., nonselective for Citrobacter spp., nonselective for Cronobacter spp., nonselective for Escherichia coli, nonselective for Klebsiella spp., nonselective for Proteus spp., nonselective for Pseudomonas spp., nonselective for Salmonella spp., nonselective for Shigella spp., nonselective for Staphylococcus spp., nonselective for Streptococcus spp., nonselective for Yersinia spp., nonselective for bacteria (General Media), nonselective for coliforms, nonselective for enterobacteriaceae

General description

Buffered peptone water being suitable as first diluent is helpful since the same homogenate can be used for detection of other microorganisms. Pre-enrichment of the culture media is performed using buffered peptone water to increase target concentration and improve sensitivity. It is also useful for the non-selective pre-enrichment of media for bacteria, in particular pathogenic members of the Enterobacteriaceae (sub-lethal damaged), from food and other material.

Application

Buffered peptone water may be used to homogenize poultry samples. It may also be used in microbial culture of Staphylococcus aureus and followed by GC-MS analysis to determine the chemical composition of galangal extraxt and HPLC analysis to isolate compounds of Indian gooseberry extract.

Preparation Note

  • Store prepared media below 8°C, protected from direct light.
  • Store dehydrated powder, in a dry place, in tightly sealed containers at 2-25°C.
Dissolve 20 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Studies with salmonellae
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

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wgk

WGK 3

Storage Class

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

动物来源培养基

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF6775
BCCD8525
BCCC9736
BCCB8496
BCBZ0769

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Protocols

食品中的肠杆菌

根据EN-ISO 8523:1991和EN-ISO 4832:1991分别对食品中肠杆菌进行检测和计数的一般指南。

Enterobacteriaceae in Food

General guidance for the detection and enumeration of Enterobacteriaceae in food according to EN-ISO 8523:1991 and EN-ISO 4832:1991, respectively.

Articles

链球菌 — 检测、鉴定、分化和培养技术概述

链球菌 — 检测、鉴定、分化和培养技术概述

假单胞菌培养基和试验

一篇关于假单胞菌属的检测、鉴定、鉴别和培养的文章。

Streptococci - Overview of Detection, Identification, Differentiation and Cultivation Techniques

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

View All Articles
Dario De Medici et al.
Applied and environmental microbiology, 69(6), 3456-3461 (2003-06-06)
The objective of this study was to develop a rapid, reproducible, and robust method for detecting Salmonella enterica serotype Enteritidis in poultry samples. First, for the extraction and purification of DNA from the preenrichment culture, four methods (boiling, alkaline lysis
H J Beckers et al.
The Journal of applied bacteriology, 62(2), 97-104 (1987-02-01)
Studies have been carried out in which growth patterns of a Salmonella sp. and competing micro-organisms, especially other Enterobacteriaceae, were followed during pre-enrichment in buffered peptone water (BPw) and subsequent selective enrichment in tetrathionate broth (TBB). Pre-enrichment cultures were inoculated
Antimicrobial and antioxidant activities of Indian gooseberry and galangal extracts.
Mayachiew, Pornpimon, and Sakamon Devahastin.
LWT--Food Science and Technology, 41, 1153-1159 (2008)
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Global Trade Item Number

SKUGTIN
77187-25KG04061833510766
77187-500G04061835571888
77187-5KG04061833661659