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90107

Sigma-Aldrich

Gram′s iodine solution

for microscopy

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Synonym(s):
Potassium Triiodide
NACRES:
MA.02

grade

for microscopy

Quality Level

product line

BioChemika

form

liquid

shelf life

limited shelf life, expiry date on the label

technique(s)

microbe id | staining: suitable

color

brown to very dark brown

refractive index

n20/D 1.335

density

1.005 g/mL at 20 °C

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

suitability

bacteria

Application

Gram’s iodine solution is used as a mordant in the Gram staining process. It is added after the primary staining step with Crystal Violet; the resultant Crystal Violet - Iodine complex imparts purple color to the cells.

Biochem/physiol Actions

Iodine(I) interacts with Crystal Violet(CV) to form large CV-I complexes within the cytoplasm and outer layers of the cell.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism.

On the Trail of Campylobacter

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Streptococci- Overview of Detection, Identification, Differentiation and Cultivation Techniques

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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