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C5070

CHAPS hydrate

Name: CHAPS hydrate
Brand: SIAL

BioXtra, ≥98% (TLC)

Synonym(s):

3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate hydrate, CHAPS

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About This Item

Empirical Formula (Hill Notation):

C₃₂H₅₈N₂O₇S · xH₂O

CAS Number:

331717-45-4

Molecular Weight:

614.88 (anhydrous basis)

NACRES:

NA.21

PubChem Substance ID:

24892750

UNSPSC Code:

12161900

MDL number:

MFCD00012116

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Properties

description

zwitterionic

Quality Level

400

product line

BioXtra

assay

≥98% (TLC)

form

powder

mol wt

614.88 g/mol (anhydrous basis)

technique(s)

protein purification: suitable, protein quantification: suitable

impurities

≤0.001% Phosphorus (P), ≤0.1% Insoluble matter, <0.04% DMF

ign. residue

≤0.1%

mp

157 °C (dec.) (lit.)

solubility

H₂O: 0.1 M, clear, colorless

anion traces

chloride (Cl^-): ≤0.05%

cation traces

Al: ≤0.0005%, Ca: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Mg: ≤0.0005%, NH₄⁺: ≤0.05%, Na: ≤0.005%, Pb: ≤0.001%, Zn: ≤0.0005%

SMILES string

O.C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)C[C@@H]4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S.H2O/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36;/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41);1H2/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-;/m1./s1

InChI key

SJCUTFKCLFLIFE-JWTJKVBLSA-N

Description

General description

CHAPS is a non-denaturing, zwitterionic sulfobetaine detergent. It is mild and solubilizes membrane proteins and receptors. It is useful for breaking protein-protein interactions. It is a derivative of the naturally occurring bile salts. It has small micellar molecular weight (6,150) and high critical micellar concentration (6 - 10 mM) allowing it to be removed from samples by dialysis.

Application

10mM CHAPS detergent has been used in the process of ion-exchange chromatography for improved purification.
0.5 w/v of CHAPS is a component of high salt buffer in protein purification alongside HEPES, NaCl and EDTA.
HEPES buffer has been used as lysis buffer in immunoprecipitation, supplemented with 1% CHAPS.

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pictograms

GHS07

signalword

Warning

hcodes

H302

pcodes

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c