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CCW4691

MRS Agar

Name: MRS Agar
Brand: SIAL

bottle capacity 250 mL, bottle filling volume 200 mL, closure type, Plastic screw cap, box of 10 bottles, for lactic acid bacteria

Synonym(s):

Lactobacillus Agar acc. to De Man, Rogosa and Sharpe, de MAN, ROGOSA and SHARPE Agar

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About This Item

NACRES:

NA.74

UNSPSC Code:

41171607

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Properties

form

solid

Quality Level

200

shelf life

12 mo. at 8‑25 °C (limited shelf life, expiry date on the label)

feature

closure type Plastic screw cap, ready-to-use

composition

agar, 14.0 g/L , dipotassium phosphate, 2.00 g/L , D-(+)-glucose, 20.0 g/L , magnesium sulfate, 0.20 g/L , manganese sulfate, 0.05 g/L , meat extract, 8.00 g/L , peptone proteose, 10.0 g/L , polysorbate 80, 1.08 g/L , sodium acetate, 5.0 g/L , tri-ammonium citrate, 2.0 g/L , yeast extract, 4.0 g/L

packaging

box of 10 bottles

storage condition

(Tightly closed)

bottle capacity

250 mL

bottle filling volume

200 mL

color

yellow-brown

pH

6.2±0.2 (25 °C)

application(s)

food and beverages
microbiology

suitability

lactic acid bacteria

Description

General description

MRS Agar is a medium used for the cultivation of lactobacilli. It is a modification of a medium based on the highly nutritious properties of tomato juice. The addition of magnesium, manganese, and acetate, together with polysorbate, provides an improved medium for the growth of lactobacilli, including very fastidious species such as Lactobacillus brevis and Lactobacillus fermentum. The quality of the peptones, in addition to the meat and yeast extracts, combine all the necessary growth factors that make MRS medium one of the best media for the cultivation of lactobacilli. As the selectivity of this medium is low and contaminants tend to grow, subculturing in a (double layer) solid medium and then in broth is recommended to increase selectivity. In many cases, growth is encouraged by incubation in a CO₂-enriched atmosphere.

Application

MRS Agar is a medium is suitable for the detection, isolation, and cultivation of lactobacilli and other lactic acid bacteria from food and beverages.

Other Notes

1 box with 10 bottles of 250 mL
Injectable cap: Plastic screw inner cap

Using syringe needles with a diameter greater than 0.8 mm is not recommended.

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Storage Class

13 - Non Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Global Trade Item Number

SKU	GTIN
CCW4691-10X200ML	04061842109678