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E7889

Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt solution

for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected

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Synonym(s):
(Ethylenedinitrilo)tetraacetic acid disodium salt, EDTA disodium salt, EDTA-Na2
Linear Formula:
[-CH2N(CH2CO2Na)CH2CO2H]2
CAS Number:
139-33-3
Molecular Weight:
336.21
Beilstein:
3822669
MDL number:
MFCD00070672
PubChem Substance ID:
24894660
NACRES:
NA.31

grade

for molecular biology

Quality Level

300

sterility

0.2 μm filtered

form

liquid

reaction suitability

reagent type: chelator

concentration

0.5 M in H2O

pH

7.5-8.5

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

[Na+].[Na+].OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O

InChI

1S/C10H16N2O8.2Na/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;/q;2*+1/p-2

InChI key

ZGTMUACCHSMWAC-UHFFFAOYSA-L

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General description

Ethylenediaminetetraacetic acid (EDTA) is a polyprotic acid. The four carboxylic acid and two amine groups with lone-pair electrons of EDTA can chelate calcium and several other metal ions.
Ethylenediaminetetraacetic acid disodium salt solution is a chelating agent suitable for molecular biology applications.

Application

Ethylenediaminetetraacetic acid disodium salt solution has been used:
  • in Tris hydrochloric acid (HCl) for digesting proteinase-K to determine the amount of DNA in the corneal sample
  • with papain to digest corneal samples for the quantification of glycosaminoglycan (GAG) content
  • in pretreatment for detaching epithelial cells grown to 80% confluency
  • to prepare stock solutions for lysis buffer
  • in the preparation of urea lysis buffer

Biochem/physiol Actions

Ethylenediaminetetraacetic acid (EDTA) is used in the treatment of patients poisoned with heavy metal ions. This permeating and sensitizing agent is also used for treating biofilm-associated conditions in dentistry, medical devices, and veterinary medicine. EDTA is currently formulated into commercially available wound dressings in human medicine to regulate matrix metalloproteinases (MMPs) and manage wound infections. It is combined with other active agents to regulate microorganisms and biofilms, including citric acid, alcohol, antibiotics, polyhexamethylene biguanide (PHMB) quaternary ammonium compounds, iodine, silver, surfactants, and other antiseptics. For hematological testing, EDTA acts as an anticoagulant due to its ability in preserving the cellular components and morphology of blood cells.

Preparation Note

Prepared in 18 megohm water with Molecular Biology Reagent EDTA (E 5134)

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H373

Precautionary Statements

P260 - P314 - P501

Hazard Classifications

STOT RE 2 Inhalation

Target Organs

Respiratory Tract

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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  1. Can a solution of EDTA disodium salt, Product E7889, be autoclaved?

    Yes, solutions of EDTA may be autoclaved.

  2. What is the pH of E7889, EDTA disodium salt solution?

    E7889 has a pH specification of 7.5 to 8.5 at 25 °C.

  3. How many divalent cations does Product E7889, EDTA disodium salt, complex with?

    EDTA forms 1:1 complex with divalent cations.

  4. What type of biological activity does Product E7889, EDTA disodium salt, exhibit?

    EDTA is a metal ion-dependent enzyme inhibitor. It may be used as a metalloprotease inhibitor at a concentration of 1-10 μm, or as a DNAse I inhibitor at a concentration of 1-10 mM.

  5. At what concentration should I use Product E7889, EDTA disodium salt, as an anticoagulant?

    The optimal concentration of EDTA to be used as an anticoagulant is 1.5 mg of EDTA disodium salt per ml of blood or 1-2 drops of a 2% EDTA solution per ml of blood.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

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