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PHR1794

Hesperidin

Name: Hesperidin
Brand: SIAL

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):

Hesperidin, 3′,5,7-Trihydroxy 4′-methoxyflavanone 7-rutinoside, Hesperetin 7-rhamnoglucoside, Hesperitin-7-rutinoside

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About This Item

Empirical Formula (Hill Notation):

C₂₈H₃₄O₁₅

CAS Number:

520-26-3

Molecular Weight:

610.56

UNSPSC Code:

41116107

NACRES:

NA.24

PubChem Substance ID:

329823618

EC Number:

208-288-1

Beilstein/REAXYS Number:

75140

MDL number:

MFCD00075663

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Properties

Quality Level

300

grade

certified reference material, pharmaceutical secondary standard

agency

traceable to Ph. Eur. Y0001203, traceable to USP 1304377

API family

hesperidin

CofA

current certificate can be downloaded

packaging

pkg of 500 mg

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

mp

250-255 °C (dec.) (lit.)

application(s)

pharmaceutical (small molecule)

format

neat

storage temp.

2-8°C

SMILES string

COc1ccc(cc1O)[C@@H]2CC(=O)c3c(O)cc(O[C@@H]4O[C@H](CO[C@@H]5O[C@@H](C)[C@H](O)[C@@H](O)[C@H]5O)[C@@H](O)[C@H](O)[C@H]4O)cc3O2

InChI

1S/C28H34O15/c1-10-21(32)23(34)25(36)27(40-10)39-9-19-22(33)24(35)26(37)28(43-19)41-12-6-14(30)20-15(31)8-17(42-18(20)7-12)11-3-4-16(38-2)13(29)5-11/h3-7,10,17,19,21-30,32-37H,8-9H2,1-2H3/t10-,17-,19+,21-,22+,23+,24-,25+,26+,27+,28+/m0/s1

InChI key

QUQPHWDTPGMPEX-QJBIFVCTSA-N

Description

General description

Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Hesperidin is a flavanone analog of diosmin, present as flavonoids in Rutaceae plants. It finds extensive use because of its phlebotonic and antioxidant properties as well as vascular protection.

This compound can be found as a component in plants of the genus: angelica, mentha, valeriana

Application

Hesperidin may be used as a pharmaceutical reference standard for the determination of the analyte in plant extracts, fruit juices and pharmaceutical formulations by various chromatography techniques.

Studies of the behavior of hesperidin and hesperetin (the aglycone) with DMPC liposomes find the aglycone penetrates more deeply and binds more strongly to the "membrane." This may explain the better bioavailability of bioflavonoid aglycones in general.

These Secondary Standards are qualified as Certified Reference Materials. These are suitable for use in several analytical applications including but not limited to pharma release testing, pharma method development for qualitative and quantitative analyses, food and beverage quality control testing, and other calibration requirements.

Biochem/physiol Actions

Flavonoid phytochemical extracted from citrus species. It is the rhamnoglucoside (rutinoside) of hesperetin.

Analysis Note

These secondary standards offer multi-traceability to the USP and EP primary standards, where they are available.

Other Notes

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

To see an example of a Certificate of Analysis for this material enter LRAC0260 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Use of multiresponse statistical techniques to optimize the separation of diosmin, hesperidin, diosmetin and hesperitin in different pharmaceutical preparations by high performance liquid chromatography with UV-DAD.

Sammani MS, et al.

Talanta, 167(2), 695-702 (2017)

Improved LC methods for the determination of diosmin and/or hesperidin in plant extracts and pharmaceutical formulations.

El-Shafae AM, et al.

Journal of Pharmaceutical and Biomedical Analysis, 26(4), 539-545 (2001)

Simultaneous reversed-phase high-performance liquid chromatographic method for the determination of diosmin, hesperidin and naringin in different citrus fruit juices and pharmaceutical formulations.

Kanaze FI, et al.

Journal of Pharmaceutical and Biomedical Analysis, 33(2), 243-249 (2003)

Global Trade Item Number

SKU	GTIN
PHR1794-500MG	04061835080304