1.0% Agarose in Tris-Borate EDTA Buffer

1% Agarose in Tris Borate EDTA (TBE buffer, pH 8.3) is a powder blend that dissolves easily in boiling water to quickly make an agarose gel for electrophoresis. Typically, 1% Agarose gel can separate DNA or RNA fragments between a range of 300 – 7,000 bp

1% Agarose gel in 1X TBE buffer may be used for:

• DNA electrophoresis
• Native RNA Electrophoresis

Foil pouches

Each packet prepares 250mL of molten agarose that is typically used to cast horizontal gels. A small electrophoresis apparatus may use 30 - 50mL, medium 100mL, and large rigs 250mL. Add nucleic acid stain before pouring the gel. For optimal results pair with pHast pack running buffer 1X TBE, pH 7.3.

Contents of one pouch, when dissolved in 250 mL of ultrapure water, will yield a 1× solution containing 89 mM Tris borate, 2 mM EDTA, and 1.0% (w/v) agarose. Certified to be DNAse, RNAse, and Nickase free. Prepare in a 1L flask. Combine pHast pack contents with 250 mL ultrapure water and mix well. Microwave on high for ~1min to boil and dissolve the agarose. Use a heat resistant glove to remove the flask every 10 - 15s to mix gently by swirling the liquid in a circle at the bottom of the flask. Repeat until the agarose is fully dissolved. Cool the flask slightly with cold water while swirling every 10 – 15s, add the stain and pour the gel. Allow agarose gel to fully cool and solidify before removing combs.

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

pHast Pack is a trademark of Sigma-Aldrich Co. LLC