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PVP40

Sigma-Aldrich

Polyvinylpyrrolidone

average mol wt 40,000

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Synonym(s):
PVP, Polyvidone, Povidone
Linear Formula:
(C6H9NO)n
CAS Number:
9003-39-8
MDL number:
MFCD00149016
PubChem Substance ID:
24899319
NACRES:
NA.25

form

powder

Quality Level

200

mol wt

average mol wt 40,000

viscosity number

29-32(lit.)

application(s)

agriculture

InChI

1S/C6H9NO/c1-2-7-5-3-4-6(7)8/h2H,1,3-5H2

InChI key

WHNWPMSKXPGLAX-UHFFFAOYSA-N

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General description

Polyvinylpyrrolidone (PVP) can form complexes with phenolics and alkaloids. The complex formation allows removal of phenolics and alkaloids from plant samples, thereby inhibiting any modification of proteins by them and any hindrance they may cause in spectrophotometric determinations of protein content. PVP might also enhances the stability of enzymes.

Application

Polyvinylpyrrolidone has been used in the purification of glutamine synthetases from plant samples. It has been used to block the PVDF (polyvinylidene difluoride) membrane and to avoid nonspecific binding.

Other Notes

Polyvinylpyrrolidone is a component of Denhardt′s Solution and is included at a concentration of 1% (w/v) in the standard 50X stock solution.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Glutamine Synthetases of Higher Plants : Evidence for a Specific Isoform Content Related to Their Possible Physiological Role and Their Compartmentation within the Leaf.
McNally SF et al.
Plant Physiology, 72, 22-22 (1983)
Angiotensin II inhibits insulin signaling in aortic smooth muscle cells at multiple levels. A potential role for serine phosphorylation in insulin/angiotensin II crosstalk.
Folli F et al.
The Journal of Clinical Investigation, 100, 2158-2158 (1997)
Quantification of the Antioxidant Activity in Salt-Stressed Tissues.
Maksimovic JJD and Zivanovic BD
Plant Salt Tolerance: Methods and Protocols, 237-237 (2012)
Structural analysis of N- and O-glycans released from glycoproteins.
Jensen PH et al.
Nature Protocols, 7, 1299-1299 (2012)
Susanna Pecchia et al.
Plants (Basel, Switzerland), 8(7) (2019-07-25)
Lupins anthracnose is a destructive seed and airborne disease caused by Colletotrichum lupini, affecting stems and pods. Primary seed infections as low as 0.01-0.1% can cause very severe yield losses. One of the most effective management strategies is the development
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Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

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