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S2255

Safranin O

Dye content ≥85 %, Powder

Synonym(s):

Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

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About This Item

Empirical Formula (Hill Notation):
C20H19ClN4
CAS Number:
477-73-6
Molecular Weight:
350.84
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24899516
EC Number:
207-518-8
MDL number:
MFCD00011759
Colour Index Number:
50240
Beilstein/REAXYS Number:
3924099
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Product Name

Safranin O, Dye content ≥85 %

form

powder

Quality Level

200

composition

Dye content, ≥85%

technique(s)

microbe id | staining: suitable

pH

10 (20 °C, 10 g/L)

solubility

water: 1 mg/mL, clear, dark red to very dark red and red purple

εmax

1250-1650 at 530-534 nm in 50% ethanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

General description

Safranin O is a cationic dye, which displays less significant metachromasia. It is used to detect any variations in articular disease. Safranin O binds specifically to polyanions. It detects the amount of proteoglycan present in cartilages. Safranin O is used as a marker for glycosaminoglycan depletion in osteoarthritis. Safranin is used as a counter stain in Gram staining.

Application

Safranin O has been used in the histological analysis of femurs. It has also been used to detect the presence of glycosaminoglycans in the inner body of meniscus structures.

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
SHBM6263
SHBL9193
SHBL8347
SHBL3227
SHBL0866

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Variability in particle degradation of four commonly employed dental bone grafts
Yang S, et al.
Clinical Implant Dentistry and Related Research, 17(5), 996-1003 (2015)
Delivery of PDGF-B and BMP-7 by mesoporous bioglass/silk fibrin scaffolds for the repair of osteoporotic defects
Zhang Y, et al.
Biomaterials, 33(28), 6698-6708 (2012)
Limitations of safranin `O?staining in proteoglycan-depleted cartilage demonstrated with monoclonal antibodies
Camplejohn KL and Allard SA
Histochemistry, 89(2), 185-188 (1988)
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Global Trade Item Number

SKUGTIN
S2255-100G04061836918552
S2255-25G04061836918576
S2255-5G04061834990680