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E7023

Sigma-Aldrich

Ethyl alcohol, Pure

greener alternative

200 proof, for molecular biology

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Synonym(s):
Ethanol, absolute alcohol, non-denatured ethanol
Linear Formula:
CH3CH2OH
CAS Number:
Molecular Weight:
46.07
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

grade

for molecular biology

Quality Level

vapor density

1.59 (vs air)

Assay

≥99.45%

form

liquid

expl. lim.

3.1-27.7 %

greener alternative product characteristics

Safer Solvents and Auxiliaries
Use of Renewable Feedstocks
Learn more about the Principles of Green Chemistry.

concentration

200 proof

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤0.2% (water)

pH

7.0 (20 °C, 10 g/L)

bp

78.3 °C

mp

-114 °C

density

0.789 g/mL at 20 °C

application(s)

agriculture

greener alternative category

InChI

1S/C2H6O/c1-2-3/h3H,2H2,1H3

InChI key

LFQSCWFLJHTTHZ-UHFFFAOYSA-N

Gene Information

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General description

This product is 200proof, non-denatured absolute ethanol (100%). This is a molecular biology grade product and is tested for the RNase and Dnase.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. Ethanol is environmentally preferable green solvent and thus it aligns with "Safer Solvents and Auxiliaries" and "Use of Renewable Feedstocks". 

Application

Pure ethyl alcohol (ethanol) has been used in the following process:
  • DNA and RNA extraction
  • array hybridization
  • ethanol sedation assay
Suitable for use in the precipitation of nucleic acids

Biochem/physiol Actions

Positive allosteric modulator of GABAA receptors, and negative allosteric modulator of NMDA glutamate receptors.

Other Notes

Sales restrictions may apply

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

55.4 °F

Flash Point(C)

13 °C

Regulatory Information

危险化学品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Is Product E7023, Ethanol 200 proof, denatured?

    No.  Product number E7023 is a nondenatured ethanol.

  3. Is Product E7023, Ethanol 200 proof, RNase and DNase free? 

    Yes. This is a molecular biology grade product and is tested for the RNase and Dnase.

  4. Can Product E7023, Ethanol 200 proof, be used for RNA extraction?

    Yes. This product is tested for use in RNA extraction.

  5. Is Product E7023, Ethanol 200 proof, 100% ethanol?

    Yes.  This product is 200 proof, absolute ethanol.  To determine the percentage of ethanol you can divide the proof number by 2.  Consequently, this product is 100% ethanol. 

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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Derek J Stefanik et al.
Nature protocols, 8(5), 892-899 (2013-04-13)
Among marine invertebrates, the starlet sea anemone Nematostella vectensis has emerged as an important laboratory model system. One advantage of working with this species relative to many other marine invertebrates is the ease of isolating relatively pure DNA, RNA and
Jaishree Garhyan et al.
BMC research notes, 6, 72-72 (2013-03-01)
Hybridization based assays and capture systems depend on the specificity of hybridization between a probe and its intended target. A common guideline in the construction of DNA microarrays, for instance, is that avoiding complementary stretches of more than 15 nucleic
Ran Lin et al.
PLoS genetics, 11(8), e1005366-e1005366 (2015-08-05)
The heavy consumption of ethanol can lead to alcohol use disorders (AUDs) which impact patients, their families, and societies. Yet the genetic and physiological factors that predispose humans to AUDs remain unclear. One hypothesis is that alterations in mitochondrial function
Gian Marco Leggio et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 39(8), 2017-2028 (2014-03-04)
Mesolimbic dopamine (DA) controls drug- and alcohol-seeking behavior, but the role of specific DA receptor subtypes is unclear. We tested the hypothesis that D3R gene deletion or the D3R pharmacological blockade inhibits ethanol preference in mice. D3R-deficient mice (D3R(-/-)) and
N Di Girolamo et al.
Stem cells (Dayton, Ohio), 33(1), 157-169 (2014-06-27)
Stem cell (SC) division, deployment, and differentiation are processes that contribute to corneal epithelial renewal. Until now studying the destiny of these cells in a living mammal has not been possible. However, the advent of inducible multicolor genetic tagging and

Protocols

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents Protocol from Cold Spring Harbor Laboratory Press.

Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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