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Merck
CN

18470

Bromothymol Blue sodium salt

suitable for microscopy

Synonym(s):

Bromthymol Blue sodium salt, 3′,3′′-dibromothymolsulfonephthalein sodium salt

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About This Item

Empirical Formula (Hill Notation):
C27H27Br2NaO5S
CAS Number:
Molecular Weight:
646.36
UNSPSC Code:
12171500
NACRES:
NA.25
PubChem Substance ID:
EC Number:
252-169-7
Beilstein/REAXYS Number:
6887926
MDL number:
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grade

Molecular Biology

form

powder

technique(s)

titration: suitable

visual transition interval

6.0-7.6, yellow to blue

suitability

suitable for microscopy

storage temp.

room temp

SMILES string

[Na+].CC(C)c1cc(c(C)c(Br)c1O)C(=C2\C=C(C(C)C)C(=O)C(Br)=C2C)\c3ccccc3S([O-])(=O)=O

InChI

1S/C27H28Br2O5S.Na/c1-13(2)18-11-20(15(5)24(28)26(18)30)23(17-9-7-8-10-22(17)35(32,33)34)21-12-19(14(3)4)27(31)25(29)16(21)6;/h7-14,30H,1-6H3,(H,32,33,34);/q;+1/p-1/b23-21+;

InChI key

NMKFVGALBGZKGW-FKWCIMQXSA-M

General description

Bromothymol blue is a pH indicator for weak acids and bases. In neutral solution, it appears bluish-green. At pH above 7.6 it appears blue, and at pH below 6.0 it appears yellow.
Bromothymol blue (BTB) is an anionic dye. It can be protonated or deprotonated to generate yellow or blue. BTB helps to make ion-pair complex, that is used to identify several pharmaceutical compounds with the help of extractive spectrophotometric methods.

Application

Suitable for use:
  • In preparations of pH indicator solutions
  • In phospholipid staining on tissues and cells prepared on microscopy slides


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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