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Merck
CN

50649

Glyoxal solution

BioReagent, ~40% in H2O (~8.8 M), Molecular Biology

Synonym(s):

Ethanedial, Oxalaldehyde

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About This Item

Linear Formula:
OHCCHO
CAS Number:
Molecular Weight:
58.04
UNSPSC Code:
12352114
NACRES:
NA.52
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1732463
Form:
liquid
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grade

Molecular Biology

Quality Level

product line

BioReagent

form

liquid

concentration

~40% in H2O (~8.8 M)

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

refractive index

n20/D 1.409

anion traces

chloride (Cl-): ≤100 mg/kg, sulfate (SO42-): ≤100 mg/kg

cation traces

Ca: ≤5 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Na: ≤300 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Zn: ≤5 mg/kg

storage temp.

2-8°C

SMILES string

[H]C(=O)C([H])=O

InChI

1S/C2H2O2/c3-1-2-4/h1-2H

InChI key

LEQAOMBKQFMDFZ-UHFFFAOYSA-N

General description

Glyoxal is a nucleic acid denaturant. It acts as a specific probe for homopurine strands of DNA.

Application

Suitable for
  • nucleic acid denaturation
  • binding of DNA/RNA to nitrocellulose membrane

Biochem/physiol Actions

Glyoxal forms stable complex with guanine residues that results in denaturation of nucleic acids.

Other Notes

May precipitate on storage; redissolve at 50-60 °C.
This form of glyoxal is composed of 3 moles of glyoxal and 2 moles of water in a relatively stable configuration.

Disclaimer

May precipitate on storage; redissolve at 50-60 °C.


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pictograms

Health hazardExclamation mark

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter



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M Vojtisková et al.
Journal of biomolecular structure & dynamics, 5(2), 283-296 (1987-10-01)
Plasmid pEJ4, which is a derivative of pUC19 containing an insert with 60-bp-long homopurine.homopyrimidine tract from sea urchin P. miliaris histone gene spacer, was studied by chemical probes of the DNA structure osmium tetroxide and glyoxal. The former probe reacts
R N Skopp et al.
Analytical biochemistry, 169(1), 132-137 (1988-02-15)
This paper describes agarose gel electrophoresis and silver staining of denatured RNAs. Glyoxal- or formaldehyde-denatured RNAs are electrophoresed in an agarose gel cast on a plastic support using an inert, low conductivity buffer. Following electrophoresis, the gel is stained with
Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
P S Thomas
Methods in enzymology, 100, 255-266 (1983-01-01)



Global Trade Item Number

SKUGTIN
50649-25ML04061832449722
50649-100ML04061838177032