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58030

Iodonitrotetrazolium chloride

Name: Iodonitrotetrazolium chloride
Brand: SIGMA

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym(s):

2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride, p-Iodonitrotetrazolium Violet, INT

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About This Item

Empirical Formula (Hill Notation):

C₁₉H₁₃ClIN₅O₂

CAS Number:

146-68-9

Molecular Weight:

505.70

UNSPSC Code:

12352102

NACRES:

NA.25

PubChem Substance ID:

57651636

EC Number:

205-676-2

Beilstein/REAXYS Number:

4093224

MDL number:

MFCD00011961

Assay:

≥97.0% (calc. on dry substance, NT)

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Properties

product line

BioReagent

Quality Level

200

assay

≥97.0% (calc. on dry substance, NT)

mp

240 °C (dec.) (lit.), ~245 °C (dec.)

solubility

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

application(s)

histology

SMILES string

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChI key

JORABGDXCIBAFL-UHFFFAOYSA-M

Description

Application

Electron acceptor for the colorimetric assay of various dehydrogenases

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.