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Sigma-Aldrich

Potassium phosphate monobasic

BioUltra, for molecular biology, anhydrous, ≥99.5% (T)

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Synonym(s):
Monopotassium phosphate, Potassium dihydrogen phosphate, prim.-Potassium phosphate
Linear Formula:
KH2PO4
CAS Number:
Molecular Weight:
136.09
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

≥99.5% (T)

form

powder or crystals

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
≤0.001% total nitrogen (N)

pH

4.0-4.5 (25 °C, 1 M in H2O)

pKa (25 °C)

(1) 2.15, (2) 6.82, (3) 12.38 (phosphoric acid)

mp

252.6 °C (lit.)

solubility

H2O: 1 M at 20 °C, clear, colorless

density

2.338 g/mL at 25 °C (lit.)

anion traces

chloride (Cl-): ≤5 mg/kg
sulfate (SO42-): ≤30 mg/kg

cation traces

Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg

λ

1 M in H2O

UV absorption

λ: 260 nm Amax: 0.050
λ: 280 nm Amax: 0.045

SMILES string

[K+].OP(O)([O-])=O

InChI

1S/K.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+1;/p-1

InChI key

GNSKLFRGEWLPPA-UHFFFAOYSA-M

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Related Categories

Application

Potassium phosphate monobasic and dibasic, are used to create phosphate buffered solutions such as potassium phosphate buffered water and saline solutions used in biological applications.

Other Notes

Component of PBS - in the isolation and analysis of nuclear RNA

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Isolation and analysis of nuclear RNA.
J R Nevins
Methods in enzymology, 152, 234-241 (1987-01-01)
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Xinyan Wu et al.
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The PIK3CA gene is frequently mutated in human cancers. Here we carry out a SILAC-based quantitative phosphoproteomic analysis using isogenic knockin cell lines containing 'driver' oncogenic mutations of PIK3CA to dissect the signalling mechanisms responsible for oncogenic phenotypes induced by
Naoyuki Okabe et al.
International journal of oncology, 46(3), 999-1006 (2015-01-15)
Personalized therapy for non‑small cell lung cancer (NSCLC), particularly lung adenocarcinoma, has recently been significantly improved by the discovery of various molecular targets. However, this has not been the case for lung squamous cell carcinoma (SCC). In the present study

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