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About This Item
Empirical Formula (Hill Notation):
C12H13NO9
CAS Number:
Molecular Weight:
315.23
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
233-753-0
MDL number:
Beilstein/REAXYS Number:
1438576
Product Name
4-Nitrophenyl β-D-glucuronide, ≥99.0% (TLC)
Quality Level
assay
≥99.0% (TLC)
form
powder or crystals
optical activity
[α]20/D −102±3°, c = 1% in ethanol
impurities
≤0.05% free 4-nitrophenol, ≤7% water
solubility
H2O: 0.1 g/mL, clear, faintly yellow
suitability
suitable as substrate for β-glucuronidase test
storage temp.
−20°C
SMILES string
O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)[N+]([O-])=O
InChI
1S/C12H13NO9/c14-7-8(15)10(11(17)18)22-12(9(7)16)21-6-3-1-5(2-4-6)13(19)20/h1-4,7-10,12,14-16H,(H,17,18)/t7-,8-,9+,10-,12+/m0/s1
InChI key
QSUILVWOWLUOEU-GOVZDWNOSA-N
General description
Chromogenic substrate for β-glucuronidase (GUS) gene detection.
Application
4-Nitrophenyl β-D-glucuronide has been used for determining the β-glucuronidase activity in urine samples, β-glucuronidase activity of bacteria in caecum content digesta and bovine liver. It has also been used to study the inhibitory effect of Phyllanthus amarus extracts on β-glucuronidase.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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