Key Documents

View All Documentation

88417

MTT Formazan

Name: MTT Formazan
Brand: SIGMA

MTT reduction end product, powder

Synonym(s):

1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan

Select a Size

Change View

About This Item

Empirical Formula (Hill Notation):

C₁₈H₁₇N₅S

CAS Number:

57360-69-7

Molecular Weight:

335.43

UNSPSC Code:

12352204

PubChem Substance ID:

329769650

NACRES:

NA.83

MDL number:

MFCD00080731

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

Product Name

MTT Formazan, MTT reduction end product

Quality Level

100

form

powder

solubility

chloroform: 10 mg/mL, clear, dark violet

SMILES string

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+

InChI key

GIFKVQOPAJDJLO-SZVLODPRSA-N

Description

Application

MTT Formazan has been used in cell viability assay.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Other Notes

Reviews: Tetrazolium salts and formazans

Still not finding the right product?

Explore all of our products under MTT Formazan

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Costunolide, an active sesquiterpene lactone, induced apoptosis via ROS-mediated ER stress and JNK pathway in human U2OS cells.

Chao Zhang et al.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 80, 253-259 (2016-05-03)

Costunolide, an active sesquiterpene lactone, is derived from many herbal medicines and it exhibits a broad spectrum of bioactivities such as anti-inflammatory, potential anti-tumor activity. Herein we assessed the anti-cancer effects of costunolide on U2OS cells and explored the underlying

Baicalein suppresses the proliferation of human cervical cancer cells via Notch 1/Hes signaling pathway.

Hu Lian et al.

Journal of cancer research and therapeutics, 15(6), 1216-1220 (2020-01-04)

Baicalein is an active compound extracted from the roots of Scutellaria baicalensis georgi, which is widely and traditionally used in the anticancer therapy. Notch signaling pathway is usually abnormally activated in kinds of human cancers. The aim of the present

Aqueous extracts of the marine brown alga Lobophora variegata inhibit HIV-1 infection at the level of virus entry into cells.

Stephan Kremb et al.

PloS one, 9(8), e103895-e103895 (2014-08-22)

In recent years, marine algae have emerged as a rich and promising source of molecules with potent activities against various human pathogens. The widely distributed brown alga Lobophora variegata that is often associated with tropical coral reefs exerts strong antibacterial

View All Related Papers

Global Trade Item Number

SKU	GTIN
88417-1G	04061833064924
88417-250MG	04061833064931