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Merck
CN

A4656

Anti-Mouse IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Goat Anti-Mouse IgG (whole molecule)−AP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
15
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biological source

goat

Quality Level

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse

should not react with

human

technique(s)

direct ELISA: 1:1,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody binds to mouse IgGs and does not cross-react with human serum proteins.

Immunogen

purified mouse IgG

Application

Goat Anti-Mouse IgG (whole molecule)-Alkaline Phosphatase antibody has been used for ELISA assays.
Immunoprecipitated 293T cells lysates were analyzed by western blot using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary antibody at 1:7500.
Surfactant Protein A was detected in bronchoalveolar fluid using alkaline phosphatase conjugated goat anti-mouse IgG as the secondary at μg/ml in TBS/Tween containing final concentration of 0.5M NaCl.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2 and 15 mM sodium azide.

Preparation Note

Adsorbed to reduce background with human samples.

Other Notes

Antibody adsorbed with human serum proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

常规特殊物品
含少量动物源组分生物产品
低风险生物材料

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Related Content


A Davies et al.
Immunology, 85(2), 220-227 (1995-06-01)
The MIC 11 antigen is expressed on human cells and is characterized by reaction with a monoclonal antibody (mAb), 16.3A5. The gene controlling MIC 11 was recently mapped to the p13 region of chromosome 11 within 500 kb of the
A G Papavassiliou et al.
The EMBO journal, 10(2), 397-406 (1991-02-01)
Regulation of herpes simplex virus (HSV) gene expression requires the synthesis of functional ICP4, a phosphoprotein that binds to several specific sites in virus DNA and acts in trans either to activate or to repress transcription of the three major
P J Simpson et al.
Circulation, 81(1), 226-237 (1990-01-01)
Pentobarbital anesthetized dogs were subjected to 90 minutes of left circumflex coronary artery (LCCA) occlusion followed by 72 hours of reperfusion. Control or anti-Mo1 (904) F(ab')2 fragments of monoclonal antibodies were administered intravenously at a dose of 1 mg/kg beginning



Global Trade Item Number

SKUGTIN
A4656-.25ML04061835805167
A4656-.5ML04061835805174
A4656-1ML04061837514234