Anti-Mouse IgG (Fc specific)–Alkaline Phosphatase antibody produced in goat

Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice . Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Purified mouse IgG, Fc fragment

Alkaline phosphatase-conjugated goat anti-mouse Fc specific antibody was used as a secondary antibody in ELISA assays at a dilution of 1:1000 in PBS/0.1% Tween and 1% BSA for 1.5 hours at 37°C. Antibody was developed using 4-nitrophenyl phosphate (Sigma) as a substrate for 30 minutes at 37°C.

Anti-Mouse IgG (Fc specific)-Alkaline Phosphatase antibody is specific for mouse IgG and mouse IgG, Fc fragment. The product does not react with mouse IgG, Fab fragment, human IgG, IgA, and IgM, or rat IgG.

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂, 50% glycerol and 15 mM sodium azide.

Adsorbed to reduce background staining with human or rat samples.

Antibody adsorbed with human IgG and rat serum proteins.

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