Human Spinal Motor Neurons

Human iPSC-Derived Spinal Motor Neurons. GFP-expressing neurons also available (BX-0101-30-1M). Required supplements for the final cell differentiation and maturation (sold separately): BX-2100-100uL, BX-2020-100uL, BX-2040-100uL. Located in the spinal cord or at the brain stem within the motor cortex, motor neurons indirectly or directly carry signals to muscles, organs, or glands from the brain. These neurons have one axon with multiple dendrites, and can be classified either as upper motor neurons, which signal between the brain and the spinal cord, or lower motor neurons, which carry signals to muscles from the spinal cord. Related diseases: ALS, SMA, Spinal Cord Injury. Primary neurotransmitter released: Acetylcholine.

Function: Spinal Motor Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture. Spinal Motor Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings. Calcein staining demonstrates the elaborate processes of the Spinal Motor Neurons in culture.

Marker Expression: Fully differentiated Spinal Motor Neurons have high neuronal purity (>90%) and consist of greater than 70% motor neurons. Labeling with the pan-neuronal marker MAP2 (green) and the motor neuron-specific marker FOXP1 (red) highlight the purity of this neuronal product.