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Merck
CN

BX-0650-30

Human Spinal Astrocytes

Human iPSC line, Fully differentiated, Healthy Male Control (no known neurological disorders), Cryopreserved

Synonym(s):

Spinal Glial Cells

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About This Item

Biological source:
human (iPSC line)
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biological source

human (iPSC line)

form

frozen liquid

packaging

vial of 1 (contains ≥1 million cells)

technique(s)

cell culture | mammalian: suitable

shipped in

liquid nitrogen

storage temp.

-140 to -196°C

General description

Human iPSC-Derived Spinal Astrocytes. Required supplements for the final cell differentiation and maturation (sold separately): BX-2600-100uL, BX-2020-100uL. Specialized glia that are the majority of the central nervous system (CNS), astrocytes do not conduct electrical signals but promote the health of the CNS through regulating the blood-brain barrier (BBB), excess neurotransmitter clearance, and maintaining ion balance, and more. Related diseases: ALS, SMA, Spinal Cord Injury.

Application

Function: Spinal Astrocytes promote mature motor neuron activity. Co-culturing motor neurons with spinal astrocytes promotes maximal calcium influx within 10 days. Energy and structural support cell for spinal neurons and immune response. Exhibit earlier and higher levels of synchronous firing when co-cultured with spinal motor neurons.

Features and Benefits

Marker Expression and Morphology: Fully differentiated Spinal Astrocytes express key astrocyte markers GFAP (green) and S100β (red) at >90% and exhibit mature process-bearing morphologies (shown with a DAPI stain).

Labelling by the spinal-specific marker HOXB4 (red) with a Hoescht stain highlights the regional specificity of the Spinal Astrocytes.


Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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