Human Striatal Medium Spiny GABAergic Neurons

Human iPSC-Derived Striatal Medium Spiny GABAergic Neurons. Required supplements for the final cell differentiation and maturation (sold separately): BX-2400-100uL, BX-2020-100uL, BX-2040-100uL. Spiny striatal GABAergic neurons are inhibitory neurons of a striatal lineage that are found in the striatum. These neurons release GABA as their primary inhibitory neurotransmitter and are important for proper neurologic function. Related diseases: Alzheimer’s Disease, Huntington’s Disease & Schizophrenia.

Calcium Influx Assays: Changes in calcium concentration are closely tied to neuronal activity as action potentials are associated with large pre-synaptic calcium influx and a notable rise in postsynaptic calcium at excitatory synapses. This can be observed experimentally by stimulating the neurons or culturing the neurons under suitable conditions to form mature networks that exhibit spontaneous oscillations. The influx of calcium can be measured using a variety of calcium-sensitive fluorescent dyes, which are commercially available. MEA Assays: Multi-electrode arrays (MEA) measure extracellular voltage changes that occur as neurons fire action potentials. These measurements reveal the firing patterns of individual neurons as well as the patterns of neuronal networks that exist in the cell culture. Such measurements are non-invasive and allow for repeated recordings.

Marker expression: Fully differentiated spiny striatal GABAergic neurons that express GABA/DARPP32/MAP2 at DIV 7 with spontaneous firing by DIV 21.