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C0858

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

Synonym(s):

COX-2, Prostaglandin H synthase 2

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About This Item

UNSPSC Code:
12352204
NACRES:
NA.32
MDL number:
MFCD00071378
Specific activity:
≥8000 units/mg protein
Assay:
≥70% (SDS-PAGE)
Recombinant:
expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells., expressed in insect cells
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Quality Level

200

recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells., expressed in insect cells

assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥8000 units/mg protein

mol wt

dimer subunit mol wt 70-74 kDa

UniProt accession no.

P35354

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTGS2(5743)

General description

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

Other Notes

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.
Tagged with a six-residue histidine sequence near the N-terminus.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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