Carbonic Anhydrase from bovine erythrocytes

Name: Carbonic Anhydrase from bovine erythrocytes
Brand: SIGMA

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

Synonym(s):

Carbonic Anhydrase from bovine erythrocytes, Carbonate Dehydratase, Carbonate Hydrolyase

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About This Item

CAS Number:

9001-03-0

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

232-576-6

MDL number:

MFCD00081467

EC Number:

4.2.1.1 (BRENDA, IUBMB)

Specific activity:

≥3,500 W-A units/mg protein

Assay:

≥75% protein basis (biuret), ≥95% (SDS-PAGE)

Biological source:

bovine erythrocytes

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Properties

biological source

bovine erythrocytes

Quality Level

200

assay

≥75% protein basis (biuret), ≥95% (SDS-PAGE)

form

lyophilized powder

specific activity

≥3,500 W-A units/mg protein

solubility

deionized water: >10 mg/mL

storage temp.

2-8°C

Description

Application

Carbonic anhydrase, from bovine erythrocytes, is used to create carbon dioxide capture systems and to research various purification techniques . Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome .

Biochem/physiol Actions

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications .

Other Notes

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)