C3545
Monoclonal Anti-Calmodulin antibody produced in mouse
clone 6D4, ascites fluid
Synonym(s):
Anti-CALM3, Anti-calmodulin
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About This Item
Conjugate:
unconjugated
Clone:
6D4, monoclonal
Application:
WB
Citations:
18
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6D4, monoclonal
contains
15 mM sodium azide as preservative
species reactivity
chicken, Dictyostelium discoideum, bovine, rat
technique(s)
western blot: 1:2,000 using a bovine brain calmodulin preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
rat ... Calm1(24242)
General description
Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11.
Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.
Immunogen
preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.
Application
Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblot
- dot blot
- immunocytochemistry
- immunoprecipitation
- immunofluorescence
Biochem/physiol Actions
Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.
The antibody recognizes native and SDS-denatured calmodulin.
These antibodies are directed against different epitopes within the calmodulin molecule.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
常规特殊物品
动物来源生物产品
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C J Pallen et al.
The Journal of biological chemistry, 259(10), 6134-6141 (1984-05-25)
Calcineurin possesses phosphatase activity towards both protein (Stewart, A.A., Ingebritsen , T.S., Manalan , A., Klee , C.B., and Cohen, P. (1982) FEBS Lett. 137, 80-84) and nonprotein substrates ( Pallen , C.J., and Wang, J.H. (1983) J. Biol. Chem.
Identification of calmodulin and MlcC as light chains for Dictyostelium myosin-I isozymes
Crawley SW, et al.
Biochemistry, 50(30), 6579-6588 (2011)
D Hulen et al.
Cell motility and the cytoskeleton, 18(2), 113-122 (1991-01-01)
Monoclonal antibodies were raised against calmodulin purified from Dictyostelium discoideum. To increase its antigenicity, the calmodulin was conjugated to keyhole limpet hemocyanin; mice were immunized with the conjugate. Hybridomas producing antibodies against calmodulin were identified by screening culture supernatants with
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C3545-.2ML | 04061838104281 |