C4483
CD152/Fc Chimera, Cytolytic from mouse
recombinant, expressed in NS.1 cells, buffered aqueous solution
Synonym(s):
CTLA4/Fc Chimera, cytolytic
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About This Item
Form:
buffered aqueous solution
Assay:
≥99% (SDS-PAGE)
Biological source:
mouse
Recombinant:
expressed in NS.1 cells
Mol wt:
97 kDa
biological source
mouse
Quality Level
recombinant
expressed in NS.1 cells
assay
≥99% (SDS-PAGE)
form
buffered aqueous solution
mol wt
97 kDa
packaging
pkg of 1 mg
storage condition
avoid repeated freeze/thaw cycles
impurities
≤1 EU/mg protein Endotoxin level (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Cd152(12477), Ctla4(12477)
General description
The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
Biochem/physiol Actions
CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.
Physical form
Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.
Preparation Note
Purified from serum-free tissue culture supernatant
Other Notes
The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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W Steurer et al.
Journal of immunology (Baltimore, Md. : 1950), 155(3), 1165-1174 (1995-08-01)
To test the hypothesis that blockade of B7-triggered costimulation by donor cells could preclude allograft rejection, we coated crude islet allograft preparations in vitro for 1 h with a murine CTLA4/Fc fusion protein. Murine CTLA4/Fc blocks the proliferative response in
Amod A Sarnaik et al.
Cancer journal (Sudbury, Mass.), 15(3), 169-173 (2009-06-27)
Metastatic melanoma is a disease associated with poor prognosis, with a median survival reported to range from 6 to 9 months. Patients who are not candidates for surgical resection have an even worse expected survival. This is largely due to
Andrew L Mellor et al.
International immunology, 16(10), 1391-1401 (2004-09-08)
Murine dendritic cells (DCs) expressing indoleamine 2,3 dioxygenase (IDO) catabolize tryptophan and can suppress T cell responses elicited in vivo. Here, we identify specific subsets of splenic (CD11c+) dendritic cells competent to mediate IDO-dependent T cell suppression following CTLA4-mediated ligation
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C4483-1MG | 04061833492239 |