C7901
Chelex® 100 sodium form
50-100 mesh (dry)
Synonym(s):
Iminodiacetic Acid Resin
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About This Item
form
beads
Quality Level
matrix
1% cross-linked polystyrene, cross-linked polystyrene beads
matrix active group
iminodiacetic acid (sodium form), paired iminodiacetate groups
matrix attachment
through methyl group to aromatic rings
particle size
50-100 mesh (dry)
pH
4-14
capacity
≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)
Application
Chelex® 100 sodium form has been used:
- for the extraction of DNA from bee samples
- to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
- to extract DNA from bacterial cultures
Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Biochem/physiol Actions
Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.
Other Notes
Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.
Legal Information
Chelex is a registered trademark of Bio-Rad Laboratories, Inc.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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