Cytochrome c Oxidase Assay Kit

Name: Cytochrome c Oxidase Assay Kit
Brand: SIGMA

sufficient for 100 tests, soluble and membrane bound mitochondria

Synonym(s):

Cytochrome c Oxidase Activity Assay Kit

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About This Item

NACRES:

NA.84

UNSPSC Code:

12161503

Usage:

sufficient for 100 tests

Storage temp.:

−20°C

Shipped in:

dry ice

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Properties

Quality Level

200

usage

sufficient for 100 tests

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COX1(4512)

Description

Application

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Biochem/physiol Actions

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Features and Benefits

Simple, optimized protocol - Obtain reproducible results without special training needs
Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
Use to analyze the intactness of mitochondrial membranes
Simple colorimetric measurement of solution