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D1626

Sigma-Aldrich

Deoxyribonucleic acid sodium salt from salmon testes

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Synonym(s):
SS-DNA, smDNA, DNA
CAS Number:
438545-06-3
MDL number:
MFCD00130922
eCl@ss:
32160414
NACRES:
NA.51

form

solid

storage temp.

2-8°C

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Application

Deoxyribonucleic acid sodium salt has been used in a study to evaluate the cytotoxicity of enzyme inhibitors and absorption enhancers in Caco-2 cells for oral delivery of salmon calcitonin. Deoxyribonucleic acid sodium salt has also been used in a study to develop a simple method for extracting DNA from Cryptosporidium oocysts.
Deoxyribonucleic acid sodium salt from salmon testes has been used in basal medium for the anaerobic growth of several Bacillus mojavensis strains It has been also used as a component of immunoprecipitation (IP) buffer for the Microplate-Based Matrix Chromatin Immunoprecipitation (ChIP).
Salmon Sperm Deoxyribonucleic acid (SS-DNA) may be used to study physicochemical interactions of DNA with binding agents, intercalation agents, modification agents, detection agents and compaction agents. Salmon sperm DNA is used to study the loading capacity of magnetic mesoporous silica nanoparticles (M-MSNs) and binding capacities of polymer nanolayer architectures. SS-DNA may be used as substrate for development of DNA detection assays.

Linkage

Formerly listed as Type III

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Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?

    The molecular weight for product D1626 has not been determined.  In general, the reference J.A.C.S., vol. 118, 10679 (1996), reports the molecular weight to be 1.3 million or around 2,000 base pairs.

  4. What is the solubility of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?

    Sigma tests solubility in water at 2 mg/mL.  To prevent shearing of the large genomic DNA, this product should be dissolved in water (1 mg/mL) with no sonication or stirring. Gentle inversion overnight at 0-4 °C is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.

  5. What is the solution stability of Product D1626, Deoxyribonucleic acid sodium salt from salmon testes (DNA)?

    Solutions of DNA have been stored successfully for several months at 4°C, in 10 mM Tris, pH 7.5 - 8.0, with 1 mM EDTA and without a bacteriostatic agent.

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The ocular surface, 15(4), 770-783 (2017-07-01)
The potential of slow-growing mycobacteria to form biofilms in human tissues contributes to the problem of establishing an effective treatment strategy. The purpose of this study was to examine new antibiotic strategies to enhance current treatment options for these infections.
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Throughout history, the yeast Saccharomyces cerevisiae has played a central role in human society due to its use in food production and more recently as a major industrial and model microorganism, because of the many genetic and genomic tools available
Marie-Louise Ainalem et al.
Langmuir : the ACS journal of surfaces and colloids, 26(7), 4965-4976 (2009-12-05)
This study shows that DNA (linearized plasmid, 4331 base pairs and salmon sperm, 2000 base pairs, respectively) adsorbs to model membranes of zwitterionic liquid crystalline phospholipid bilayers in solutions containing divalent Ca(2+) cations, and also in solutions containing monovalent Na(+).
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