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E3889

Sigma-Aldrich

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

for molecular biology, ≥97.0%

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Synonym(s):
EGTA, Egtazic acid, Ethylene-bis(oxyethylenenitrilo)tetraacetic acid, Glycol ether diamine tetraacetic acid
Linear Formula:
[-CH2OCH2CH2N(CH2CO2H)2]2
CAS Number:
67-42-5
Molecular Weight:
380.35
Beilstein:
1717370
EC Number:
200-651-2
MDL number:
MFCD00004291
PubChem Substance ID:
24894530
NACRES:
NA.31

grade

for molecular biology

Assay

≥97.0%

form

powder

reaction suitability

reagent type: chelator

loss

≤1.0% loss on drying

mp

241 °C (dec.) (lit.)

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

OC(=O)CN(CCOCCOCCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C14H24N2O10/c17-11(18)7-15(8-12(19)20)1-3-25-5-6-26-4-2-16(9-13(21)22)10-14(23)24/h1-10H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24)

InChI key

DEFVIWRASFVYLL-UHFFFAOYSA-N

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Application

Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid has been used:
  • in Dulbecco′s phosphate-buffered saline (DPBS) to induce the weakening of cell-to-cell junctions
  • as an extracellular calcium chelator to study the role of calcium in axonal mitochondria degeneration
  • as a component in lysis buffer for lysing mouse mucosal cells for western blot analysis

A chelating agent useful for the determination of calcium in the presence of magnesium.

Biochem/physiol Actions

Ethylene glycol tetraacetic acid (EGTA) can chelate calcium (Ca2+) at a ratio of 1:1. It serves as an anti-coagulant when dissolved at 1 g per 100 ml of blood. EGTA can prevent the vesicular release in the nanodomain of single Ca2+ channels. This metal chelator can inhibit the activity of pseudolysin.
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid A is a chelating agent used for the determination of calcium in the presence of magnesium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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