E5134
for molecular biology
99.0-101.0% (titration)
powder
reagent type: chelator
electrophoresis: suitable
≤0.005% Insolubles
≤0.1% Nitrilotriacetic acid (NTA)
248 °C (dec.) (lit.)
3 M NaOH: 1.6 g/10 mL
Fe: ≤0.005%
Pb: ≤0.002%
DNase, RNase, NICKase and protease, none detected
room temp
O.O.O.O.[Na+].[Na+].[Na+].[Na+].OC(=O)CN(CCN(CC([O-])=O)CC([O-])=O)CC(O)=O.OC(=O)CN(CCN(CC(O)=O)CC([O-])=O)CC([O-])=O
1S/C10H16N2O8.2Na.2H2O/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;;;;/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);;;2*1H2/q;2*+1;;/p-2
OVBJJZOQPCKUOR-UHFFFAOYSA-L
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Warning
Acute Tox. 4 Inhalation - STOT RE 2 Inhalation
Respiratory Tract
11 - Combustible Solids
WGK 2
Not applicable
Not applicable
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Yes.
Yes. A 0.5 M stock solution at pH 8.5 is stable for months at 2-8°C.
The optimal concentration is 1.5 mg per mL of blood.
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Ask a Scientist here.
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.
Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition
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