E7510
Earle′s Balanced Salt Solution 10x
Without sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Earle’s Balanced Salts, EBSS
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About This Item
Quality Level
sterility
sterile-filtered
form
liquid
concentration
10 ×
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
components
glucose: 10.0 g/L
NaHCO3: no
phenol red: 0.11 g/L
shipped in
ambient
storage temp.
room temp
General description
Earle′s Balanced Salt Solution 10x is a saline solution with physiological pH. It is useful in inducing autophagy and promotes Bacillus Calmette-Guérin (BCG)-induced dendritic cells (DCs) maturation.
Application
Earle′s Balanced Salt Solution 10x has been used:
- as an additive to the nutritive medium sheep nematode Trichostrongylus colubriformis eggs
- in the swim-up method to prepare human sperm sample
- in the steady-state medium to culture bovine primary mammary epithelial cells
Features and Benefits
- Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.
- Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism.
- When combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism.
- Provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).
Preparation Note
Supplement with 2.2 g/L sodium bicarbonate at 1×.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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