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F6636

Nutrient Mixture F-12 Ham

Name: Nutrient Mixture F-12 Ham
Brand: SIGMA

Coon′s Modification, with ʟ-glutamine and zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

UNSPSC Code:

12352207

NACRES:

NA.75

MDL number:

MFCD00217417

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Properties

Product Name

Nutrient Mixture F-12 Ham, Coon′s Modification, with L-glutamine and 0.863 mg/L zinc sulfate, without sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | hybridoma: suitable, cell culture | mammalian: suitable

components

phenol red: 0.00125 g/L
L-glutamine: 0.292 g/L
sodium pyruvate: 0.22 g/L
glucose: 1.802 g/L

shipped in

ambient

storage temp.

2-8°C

Description

Application

Coon's modification of Ham's F-12 was developed for culturing hybrid cells that were produced by viral fusion. The modification consists of doubling the amino acids and pyruvate and including ascorbic acid. The salt concentrations have also been altered. The formula contains 0.863 mg/L zinc sulfate, which may render it unsuitable for culturing mouse L-cells.

Preparation Note

Formulated to contain 11.5 grams of powder per liter of medium.

Supplement with 2.68 g/L sodium bicarbonate.

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Protocols

粉末培养基制备说明

粉末培养基及盐混合物极具吸湿性，应注意防潮。

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.