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Merck
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FA20

Fructose Assay Kit

sufficient for 20 assays

Synonym(s):

Fructose Test Kit

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About This Item

UNSPSC Code:
12164500
EC Number:
200-333-3
NACRES:
NA.84
Technique(s):
photometry: suitable
Usage:
sufficient for 20 assays
Storage temp.:
2-8°C
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usage

sufficient for 20 assays

Quality Level

analyte chemical class(es)

sugars (fructose)

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

This kit is for the quantitative, enzymatic determination of fructose in food and other materials.Fructose is phosphorylated by adenosine triphosphate (ATP) in the reaction catalyzed by hexokinase. Fructose 6-phosphate is converted to glucose 6-phosphate by phosphoglucose isomerase (PGI). Glucose-6-phosphate (G6P) is then oxidized to 6-phosphogluconate in the presence of nicotinamide adenine dinucleotide (NAD) in the reaction catalyzed by glucose-6-phosphate dehydrogenase (G6PDH). During this oxidation, an equimolar amount of NAD is reduced to NADH. The consequent increase in absorbance at 340 nm is directly proportional to fructose concentration.


Kit Components Only

Product No.
Description

  • fructose standard 500 mg

  • glucose assay reagent 1 mL/vial

  • phosphoglucose isomerase 1 mL/vial

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

wgk

WGK 3

Storage Class

11 - Combustible Solids

Regulatory Information

常规特殊物品

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Beuter, H.O.
Methods of Enzymatic Analysis, 6, 321-321 (1985)
Official Methods of Analysis of the AOAC, 28-28 (1995)
Mengxuan Fang et al.
Analytical and bioanalytical chemistry, 412(10), 2339-2351 (2020-02-02)
Fatty acids are a major source of structural diversity within the lipidome due to variations in their acyl chain lengths, branching, and cyclization, as well as the number, position, and stereochemistry of double bonds within their mono- and poly-unsaturated species.



Global Trade Item Number

SKUGTIN
FA20-1KT04061833622292
G3293-50ML04061837904998
G3293-20ML04061837904981
F2793-500MG04061833613313