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Merck
CN

G1135

L-Glutamic acid γ-(4-nitroanilide)

≥98% (HPLC), γ-glutamyl transpeptidase substrate, powder

Synonym(s):

L-γ-Glutamyl-p-nitroanilide, L-Glutamic acid 5-(4-nitroanilide)

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About This Item

Linear Formula:
C11H13N3O5
CAS Number:
Molecular Weight:
285.25
NACRES:
NA.83
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
230-748-5
MDL number:
Beilstein/REAXYS Number:
2818758
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Product Name

L-Glutamic acid γ-(4-nitroanilide), γ-glutamyl transpeptidase substrate

Quality Level

assay

≥98% (HPLC)

form

powder

solubility

formic acid: 50 mg/mL, clear to slightly hazy

storage temp.

2-8°C

SMILES string

N[C@@H](CCC(=O)Nc1ccc(cc1)[N+]([O-])=O)C(O)=O

InChI

1S/C11H13N3O5/c12-9(11(16)17)5-6-10(15)13-7-1-3-8(4-2-7)14(18)19/h1-4,9H,5-6,12H2,(H,13,15)(H,16,17)/t9-/m0/s1

InChI key

WMZTYIRRBCGARG-VIFPVBQESA-N

General description

Substrate for γ-glutamyl transpeptidase


Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Gololobov MYu et al.
The Biochemical journal, 304 ( Pt 3), 869-876 (1994-12-15)
Acyl-transfer catalysed by gamma-glutamyltranspeptidase from bovine kidney was studied using gamma-L- and gamma-D-Glu-p-nitroanilide as the donor and GlyGly as the acceptor. The transfer of the gamma-Glu group to GlyGly was shown to be accompanied by transfer of the gamma-Glu group
The reaction of oxidized and reduced glutathione (with some amino-acids) and L-gamma-glutamyl p-nitroanilide with sheep kidney tissue homogenate. An examination of the reaction products by paper chromatography.
P C Nicholas et al.
Biochemical Society transactions, 19(1), 50S-50S (1991-02-01)
Long-Liu Lin et al.
Applied microbiology and biotechnology, 73(1), 103-112 (2006-07-20)
A truncated gene from Bacillus lichenifromis ATCC 27811 encoding a recombinant gamma-glutamyltranspeptidase (BLrGGT) was cloned into pQE-30 to generate pQE-BLGGT, and the overexpressed enzyme was purified from the crude extract of IPTG-induced E. coli M15 (pQE-BLGGT) to homogeneity by nickel-chelate



Global Trade Item Number

SKUGTIN
G1135-1G04061833625149
G1135-5G04061833625156