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Merck
CN

G4410

Griess reagent (modified)

Synonym(s):

Greiss, nitrite assay

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About This Item

UNSPSC Code:
12352200
EC Number:
215-981-2
NACRES:
NA.32
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form

powder

Quality Level

usage

10 g

General description

Griess reagent is based on Griess reaction. The components include naphthylethylenediamine dihydrochloride suspended in water and sulphanilamide in phosphoric acid. This reagent reacts with nitrite in samples to form a purple azo product, absorbance of which is measured at 546 nm.

Application

Griess reagent (modified) has been used:
  • in the nitrite assay of primary spinal cord glial cells stimulated with supernatant of damaged sensory neuron (SDSN)
  • for measuring nitrite/nitrate oxidized from nitric oxide in stimulated chondrocytes
  • for measuring nitric oxide (NO) degradation in RAW264.7 macrophage cells
  • to measure the concentration of nitric oxide(NO) in the conditioned media from cultured macrophages (MΦs)
  • to determine the relative levels of NO released by BEAS-2B bronchial cells in the culture supernatants


pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1

Storage Class

8A - Combustible corrosive hazardous materials

wgk

WGK 2



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Hyung Soo Han et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(10), 3921-3928 (2002-05-23)
Mild hypothermia is neuroprotective, but the reasons are not well known. Inflammation contributes to ischemic damage; therefore, we examined whether the protection by hypothermia may be attributable to alterations in the inflammation. We examined whether hypothermia might alter the inflammatory
Praveen Papareddy et al.
The Journal of biological chemistry, 285(36), 28387-28398 (2010-07-02)
Tissue factor pathway inhibitor (TFPI) inhibits tissue factor-induced coagulation, but may, via its C terminus, also modulate cell surface, heparin, and lipopolysaccharide interactions as well as participate in growth inhibition. Here we show that C-terminal TFPI peptide sequences are antimicrobial
T Tikka et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 21(8), 2580-2588 (2001-04-18)
Minocycline, a semisynthetic tetracycline derivative, protects brain against global and focal ischemia in rodents. We examined whether minocycline reduces excitotoxicity in primary neuronal cultures. Minocycline (0.02 microm) significantly increased neuronal survival in mixed spinal cord (SC) cultures treated with 500



Global Trade Item Number

SKUGTIN
G4410-10G04061835573080